In female mammals, Bartholin's glands open into the:. Vaginal Discharge Teen Health Source The Bartholin's (BAHR toe linz) glands are located on each side of the vaginal opening. These glands secrete fluid that helps lubricate the vagina. Sometimes the openings of these glands become Arousal, lubrication AND the vestibular glands. The greater and lesser vestibular glands or bartholin and skene glands secrete fluid which provides lubrication. However, this is not the primary source of vaginal lubrication. Can cycling affect my vaginal health? VIDA Wellness and Beauty